Escherichia coli, enteroinvasive

Escherichia coli, enteroinvasive

MATERIAL SAFETY DATA SHEET - INFECTIOUS SUBSTANCES

SECTION I - INFECTIOUS AGENT

NAME: Escherichia coli, enteroinvasive

SYNONYM OR CROSS REFERENCE: EIEC, bacillary dysentery

CHARACTERISTICS: Gram negative rod; motile, aerobic; serotyping

SECTION II - HEALTH HAZARD

PATHOGENICITY: Disease localized primarily in the colon (invasion of epithelial cells of colon, similar to dysentery; shigella-like disease); fever; mucoid, occasionally bloody diarrhea; generally self-limiting; most severe form may result in hypotension with severe toxemia; sometimes associated with food poisoning

EPIDEMIOLOGY: Usually sporadic, particularly in under-developed countries; may cause common source outbreaks

HOST RANGE: Human

INFECTIOUS DOSE: Appears to have low infectious dose, may be similar to that of Shigella spp.,10 organisms by ingestion

MODE OF TRANSMISSION: Fecal-oral route; fecal contamination of water, food or fomites; poor sanitation and hygiene

INCUBATION PERIOD: 12-72 hours

COMMUNICABILITY: Communicable during duration of fecal excretion (several weeks)

SECTION III - DISSEMINATION

RESERVOIR: Infected persons

ZOONOSIS: No

VECTORS: None

SECTION IV - VIABILITY

DRUG SUSCEPTIBILITY: Sensitive to ampicillin

SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to many disinfectants - 1% sodium hypochlorite, 70% ethanol, glutaraldehyde, iodines, phenolics, formaldehyde

PHYSICAL INACTIVATION: Inactivated by moist heat (121° C for at least 15 min) and dry heat (160-170° C for at least 1 hour

SURVIVAL OUTSIDE HOST: Survives well in contaminated feces, food, soil or water

SECTION V - MEDICAL

SURVEILLANCE: Monitor for symptoms; confirm by examination of stool specimens; demonstration of invasiveness of isolates in tissue culture

FIRST AID/TREATMENT: Therapy generally not necessary as illness is short-lived; electrolyte fluid therapy if excessive diarrhea; antibiotic therapy in severe cases

IMMUNIZATION: None

PROPHYLAXIS: Not usually administered

SECTION VI - LABORATORY HAZARDS

LABORATORY-ACQUIRED INFECTIONS: 3 reported cases of laboratory infections with E. coli

SOURCES/SPECIMENS: Feces; contaminated food, water, fomites

PRIMARY HAZARDS: Ingestion

SPECIAL HAZARDS: None

SECTION VII - RECOMMENDED PRECAUTIONS

CONTAINMENT REQUIREMENTS: Biosafety level 2 practices containment equipment and facilities for activities involving cultures and infected clinical materials

PROTECTIVE CLOTHING: Laboratory coat; gloves when contact with infectious materials is unavoidable

OTHER PRECAUTIONS: Good personal hygiene and frequent handwashing

SECTION VIII - HANDLING INFORMATION

SPILLS: Allow aerosols to settle; wearing protective clothing, gently cover spill with absorbent paper towel and apply 1% sodium hypochlorite, starting at perimeter and working towards the centre; allow sufficient contact time (30 min) before clean up

DISPOSAL: Decontaminate before disposal; steam sterilization, chemical disinfection

STORAGE: In sealed containers that are appropriately labelled

SECTION IX - MISCELLANEOUS INFORMATION

Date prepared: January, 2000

Prepared by: Office of Laboratory Security, PHAC

Although the information, opinions and recommendations contained in this Material Safety Data Sheet are compiled from sources believed to be reliable, we accept no responsibility for the accuracy, sufficiency, or reliability or for any loss or injury resulting from the use of the information. Newly discovered hazards are frequent and this information may not be completely up to date.

Copyright © Health Canada, 2001

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